| Mill Creek Motor Freight Ltd | Ayr, Ontario | 225 | 286 |
| 2044844 Ontario Inc | Ayr, Ontario | 130 | 146 |
| J & R Hall Transport Inc | Ayr, Ontario | 110 | 197 |
| Transfrt Mcnamara Inc | Ayr, Ontario | 84 | 82 |
| Kapila Transport Inc | Ayr, Ontario | 49 | 49 |
| Patco Equipment Inc | Ayr, Ontario | 35 | 64 |
| Equipment Express Inc | Ayr, Ontario | 33 | 27 |
| Beyond Transportation Inc | Ayr, Ontario | 32 | 33 |
| T E A M Logistics Systems Inc | Ayr, Ontario | 32 | 36 |
| Patco Transportation Inc | Ayr, Ontario | 30 | 30 |
| Wjs Transport Inc | Ayr, Ontario | 30 | 32 |
| Dlh Transportation Inc | Ayr, Ontario | 28 | 28 |
| Hope Systems Inc | Ayr, Ontario | 28 | 32 |
| Smartway Freight Systems Inc | Ayr, Ontario | 28 | 30 |
| Heritage Truck Lines Inc | Ayr, Ontario | 27 | 31 |
| Jbt Transport Inc | Ayr, Ontario | 27 | 31 |
| Ks Benipal Transport Inc | Ayr, Ontario | 26 | 35 |
| 2475222 Ontario Inc | Ayr, Ontario | 25 | 56 |
| Bright Water Services Inc | Ayr, Ontario | 13 | 12 |
| Rw Maus Transport Inc | Ayr, Ontario | 7 | 8 |
| 11591185 Canada Inc | Ayr, Ontario | 5 | 5 |
| Icon Motors Ltd | Ayr, Ontario | 4 | 6 |
| Kyubi Transport Inc | Ayr, Ontario | 4 | 4 |
| 11412159 Canada Inc | Ayr, Ontario | 3 | 2 |
| Aevitas Inc | Ayr, Ontario | 2 | 1 |
| Dhanoa Trans Inc | Ayr, Ontario | 2 | 4 |
| Jorge D Enterprises Ltd | Ayr, Ontario | 2 | 1 |
| Mm Bros Dhanoa Inc | Ayr, Ontario | 2 | 2 |
| Bnk Transport Ltd | Ayr, Ontario | 1 | 1 |
| Ken Koehler | Ayr, Ontario | 1 | 1 |
| Team Vincent Motorsport Inc | Ayr, Ontario | 1 | 1 |
| Great Goose Logistics Inc | Ayr, Ontario | 0 | — |
| Heritage Freight Forwarding Ltd | Ayr, Ontario | 0 | — |
| Icon Transportation Logistics Ltd | Ayr, Ontario | 0 | — |
| Mill Creek Motor Freight Limited | Ayr, Ontario | 0 | — |